Biom2005

6.1. Acknowledgements

Any acknowledgements should appear first at the end of your article prior to your Declaration of Conflicting Interests (if applicable), any notes and your References.

All contributors who do not meet the criteria for authorship should be listed in an ‘Acknowledgements’ section. Examples of those who might be acknowledged include a person who provided purely technical help, writing assistance, or a department chair who provided only general support. Authors should disclose whether they had any writing assistance and identify the entity that paid for this assistance.

From http://www.uk.sagepub.com/msg/nej.htm#ACKNOWLEDGMENTS

Biom2005 HepG2 Student Work

Hep G2 cells are epithelial cells found in the liver, the cells secrete a variety of major plasma proteins including albumin, ά 1 – antitrypsin, transferrin andplasminogen. The cells respond well to stimulation by human growth hormone (HGH). The cells are free from any known hepatotropic viral agents and due to their functional role in cholesterol and triglyceride metabolism they are beneficial for studying the central role of the liver.

Since the introduction of the Hep G2 cell line in1979 more than 250 studies have been published using this resource (Javitt N B 1990). The high proportion of liver specific proteins found in Hep G2 cells make it useful as a model of the human liver.

The optimum culture medium for Hep G2 cells has been found to be 2% Glutamine, 1% non essential amino acids and 10% Foetal bovine serum (JavittN B 1990)

Cultures of Hep G2 cells can be purchased from Sigmaaldrich.com and start from £250 a culture. The culture will be shipped in dry ice.

 

 

Bibliography

1.

Javitt, N B, 1990. Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol and bile acids. New York. The Federation of American Societies for Experimental Biology.

2.

Sigma Aldrich, (2013) Sigma-Aldrich, http://www.sigmaaldrich.com/catalog/product/sigma/85011430?lang=en®ion=GB, 01/10/2013